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Dog DNA sample collection kit is used for dog DNA sample collection, which is usually the first step in a longer laboratory process. DNA extraction is an important part of the process because the DNA needs to be purified and separated from proteins and other cellular contaminants. It is based on a combination of different physical and chemical methods. DNA can be found in everything living and therefore it is possible to use anything living as a source of DNA for extraction. The most common sources of DNA used for cat or dog DNA sample collection are blood and buccal swab and they differ among each other by the quality and quantity of extracted DNA. Between those two DNA sources, a buccal swab may be easier to obtain in a non-invasive manner, than the blood sample. In AnimaLabs’ laboratory, for genetic tests for dogs, dog DNA sample collection kits for blood and buccal swab are being used.
Based on provided charts, it can be easily concluded that the blood is the best source of DNA for DNA isolation. However, the buccal swab sample still provides sufficient quality and quantity of DNA for further processing and DNA testing.
The Source of the DNA in the buccal swab are the mucosa cells, which are situated on the inside of a cheek. There is no DNA in the saliva itself, but in the cellular content present in it. Buccal swab collection has many advantages. It can be easily performed using a collection swab, it provides sufficient DNA for the PCRs and long-term storage. On the other hand, DNA yield and its quality is worse when isolating from the buccal swab, because of possible food leftovers and smaller number of cells than in a blood sample.
The Source of the DNA in the blood are the white blood cells. Red blood cells cannot be a source of DNA, since they lack nuclei.
The buccal swab can be taken using a collection swab from a dog DNA sample collection kit provided by AnimaLabs or your own sterile collection swab. It is important that the swab does not come in contact with anything other than your pet’s mouth, in order to prevent contaminations with foreign DNA. Be careful also not to touch the swab with your own hands. After taking the swab sample, it is vital to let is dry on air. By drying the buccal swab on air, bacterial contaminations and degradation of the DNA is avoided. Do not dry the swab by blowing on it.
Once you have taken the buccal swab sample, do not return it into its plastic package but place it in the AnimaLabs envelope, according to the identification number of the sample and animal. Envelope with sample should be sent the same day, but if you are not in possibility to do so, store the envelope in the fridge and send as soon as you are able to.
When it comes to blood samples, you must be ready to send the samples the same day as they are collected. Until sending it, the sample should be stored at 4°C. The fresher the sample, the more successful the testing is.
For more detailed information on buccal and blood collection instructions, look here or watch video.
In case of using your own collection swab or sending blood sample, in the cart use code 'shipping' in the coupon number field as you check out from AnimaLabs webshop, and the price will be reduced for the price of the dog DNA sample collection kit.