In Felids sex can be identified by DNA testing. Two high specific loci for Felidae family and domestic cat (Felis silvestris) are tested by PCR. This sex-specific test offers non-invasive, fast and accurate sex identification in all cat breeds.
Pilgrim, K.L., Mckelvey, K.S., Riddle, A.E., and Schwartz, M.K. (2005). Felid sex identification based on noninvasive genetic samples. Molecular Ecology Notes 5, 60–61.